PEPC丨磷酸烯醇丙酮酸羧化酶实用方案丨新手福利

Cat#：AS09-458

应用类型：Immunolocalization (IL), WB

AS09 458 |克隆性：多克隆|宿主：兔|反应性：A。科摩索斯，A。拟南芥。睫状体。加亚纳，C。维利亚，H。斯沃格，J。库卡斯，L。褐飞虱。马克西姆斯，M。结晶蛋白。塔巴库姆，O。萨蒂瓦，P。药。色拉图姆，P。甘蔗属strobus杂交克隆C91-301，S。拉纳塔，S。落叶松。双色，聚囊藻PCC 6803，三角褐指藻（菌株CCAP 1055/1），T。魏斯弗洛吉，Z。梅斯，Z。灰长臂猿

艾美捷PEPC丨磷酸烯醇丙酮酸羧化酶 应用实例：

10µg从拟南芥wt叶组织（Atn非衰老叶）、拟南芥wt叶组织（Ats衰老叶）、红松针叶组织（PS0、PS36）中用1 M Tris HCl、pH 6.8、10%SDS、15%蔗糖新鲜提取的总蛋白，在10%Bis-Tris Nupage Novex凝胶（120 V/45 min，使用MES缓冲系统）上分离0.5 DTT和在75°C下变性5 min，并在30 min内用聚偏氟乙烯（PVDF）吸附。搅拌45分钟/RT，用5%脱脂牛奶阻断印迹。在含有2%脱脂乳的TBS中搅拌，或在/4°C下搅拌，以1:1000的稀释度在一级抗体中孵育1h/RT。倾析抗体溶液，在RT条件下搅拌，在TBS中短暂冲洗印迹两次，持续10分钟。在Agrisera匹配二级抗体（抗兔IgG-辣根过氧化物酶结合物，AS09 602）中培养印迹，稀释至1:75 000 in，搅拌1h/RT。如上所述清洗印迹，并使用化学发光检测进行显影。曝光时间为40秒。

参考资料：

Jiang et al. (2017). Development of an Efficient Protein Extraction Method Compatible with LC-MS/MS for Proteome Mapping in Two Australian Seagrasses Zostera muelleri and Posidonia australis. Frontiers in Plant Science, doi: 10.3389/fpls.2017.01416.

Liu et al. (2017). Plasma Membrane CRPK1-Mediated Phosphorylation of 14-3-3 Proteins Induces Their Nuclear import to Fine-Tune CBF Signaling during Cold Response. Mol Cell. 2017 Apr 6;66(1):117-128.e5. doi: 10.1016/j.molcel.2017.02.016.

Ribeiro et al. (2017). Increased sink strength offsets the inhibitory effect of sucrose on sugarcane photosynthesis. J Plant Physiol. 2017 Jan;208:61-69. doi: 10.1016/j.jplph.2016.11.005.

Shen et al. (2016). The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice. Rice (N Y). 2016 Dec;9(1):20. doi: 10.1186/s12284-016-0094-5. Epub 2016 May 10.

Ishikawa et al. (2016). NDH-Mediated Cyclic Electron Flow Around Photosystem I is Crucial for C4 Photosynthesis. Plant Cell Physiol. 2016 Aug 6. pii: pcw127. [Epub ahead of print]

Shen et al. (2015). Overexpression of maize phosphoenolpyruvate carboxylase improves drought tolerance in rice by stabilization the function and structure of thylakoid membrane. Photosynthetica, September 2015, Volume 53, Issue 3, pp 436-446.

Foley et. al (2015). Analysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approaches. BMC Plant Biology 2015, 15:106 doi:10.1186/s12870-015-0485-6.

Agrisera公司提供的植物领域的抗体涵盖拟南芥、莱茵衣藻、蓝藻、松柏类、硅藻、大豆、大麦、蒺藜苜蓿等等。艾美捷科技是Agrisera的中国代理商，为科研工作者提供优质的PEPC丨磷酸烯醇丙酮酸羧化酶。